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KMID : 0382619820020020347
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Hanyang Journal of Medicine
1982 Volume.2 No. 2 p.347 ~ p.359
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Activities of Hepatic and Serum 5´-Nucleotidase in Liver Disorders
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Abstract
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In order to evaluate mechanisms by which serum 5´-nucleotidase activity was increased in liver disorders, activities of 5´-nucleotidase bound to the membrane or solubilized by sodium dodecyl sulfate (SDS) were determined in homogenates and subcellular fractions of livers from rats treated with phenobarbital and CC14. Also measured was the activity of hepatic 5´-nucleotidase solubilized by SDS and separated by DEAE-cellulose chromatography in liver damaged rats, and the results were compared with those in normal rats.
1) Although the activity of serum 51-nucleotidase was significantly increased in rats with hepatotoxicity induced by phenobarbital and CCl4, the activity of the hepatic enzyme was not changed. These results indicate that the enzyme might be released from the damaged liver into the blood without showing any significant fall in the activity of the hepatic enzyme.
2) In Tris-and SDS-liver cell fractions (103xg and 104xg fractions) prepared from liver of rats with CC14 hepatotoxicity, ratio of 5´-nucleotidase activity in the supernatant to that in the precipitates was observed to be increased as compared with that of normal rats. The result suggested that in rats with CC14 hepatotoxicity the activity of hepatic 5´nucleotidase bound to the membrane was decreased and the activity of free or intermediate form of the enzyme was increased.
3) The total activity of hepatic 5´-nucleotidase separated by DEAE-cellulose chromatography in rats with CC14 hepatotoxicity was greater than that in normal rats. This result confirmed that the activity of free form of hepatic 5´-nucleotidase was increased in rats with CC14 hepatotoxicity.
It can be concluded with the results obtained in the present study that an increase in the, serum 5´-nucleotidase observed in rats with CC14 hepatotoxicity was due to the release into the blood of the free form of the enzyme that was found to be increased in the liver of rats with CC14 hepatotoxicity.
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